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Registro Completo |
Biblioteca(s): |
Embrapa Rondônia. |
Data corrente: |
22/09/2016 |
Data da última atualização: |
06/10/2016 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
SANTOS, M. R. A. dos; SOUZA, C. A. de. |
Afiliação: |
MAURICIO REGINALDO ALVES DOS SANTOS, CPAF-Rondonia; Carolina Augusto de Souza. |
Título: |
Dedifferentiation of Leaf Cells and Growth Pattern of Calluses of Capsicum annuumcv. Etna. |
Ano de publicação: |
2016 |
Fonte/Imprenta: |
Australian Journal of Basic and Applied Sciences, v. 10, n. 12, p. 362-368, 2016. |
Idioma: |
Inglês |
Conteúdo: |
Background:In vitrocell suspension cultivation systems have been largely reported assafe and standardized methods for production of secondary metabolites with medicinaland agricultural interest.Capsicum annuumis one of the most widely grown vegetablein the world and its biological activities have been demonstrated against insects, fungi,bacteria and other groups of organisms. The determination of procedures for thededifferentiation of cells into callus cells and the subsequent study of the callus growthpattern are necessary for the establishment of cellsuspensions and also to subsidizestudies regarding the bioactivity of its secondarymetabolites. To date, no study hasdescribed the development of protocols for callus induction inC. annuumL. cv. Etna. Objective:The objective of this study was to establish a protocol for dedifferentiationof leaf cells of the cultivarC. annuumcv. Etna and to determine the growth pattern ofthe calluses with a focus on the deceleration phase, when the callus cells must besubcultured into a liquid medium in order to establish cell suspension cultivationsaiming at the production of secondary metabolites.Results:The treatment that resultedin the highest %CI, ACCC and callus weight was thecombination of 4.52 μ M 2,4-D +0.44 μ M BA. The calluses produced were friable andwhitish and their growth patternfollowed a sigmoid shape. The deceleration phase started on the 23rdday of cultivation.Conclusion:Callus induction in leaf explants ofC. annuumcv. Etnacan be achieved inMS medium supplemented with 4.52 μ M 2,4-D + 0.44 μ MBA, which results in highcellular proliferation; in order to start a cell suspension culture, callus cells on the 23rdday of culture should be used. MenosBackground:In vitrocell suspension cultivation systems have been largely reported assafe and standardized methods for production of secondary metabolites with medicinaland agricultural interest.Capsicum annuumis one of the most widely grown vegetablein the world and its biological activities have been demonstrated against insects, fungi,bacteria and other groups of organisms. The determination of procedures for thededifferentiation of cells into callus cells and the subsequent study of the callus growthpattern are necessary for the establishment of cellsuspensions and also to subsidizestudies regarding the bioactivity of its secondarymetabolites. To date, no study hasdescribed the development of protocols for callus induction inC. annuumL. cv. Etna. Objective:The objective of this study was to establish a protocol for dedifferentiationof leaf cells of the cultivarC. annuumcv. Etna and to determine the growth pattern ofthe calluses with a focus on the deceleration phase, when the callus cells must besubcultured into a liquid medium in order to establish cell suspension cultivationsaiming at the production of secondary metabolites.Results:The treatment that resultedin the highest %CI, ACCC and callus weight was thecombination of 4.52 μ M 2,4-D +0.44 μ M BA. The calluses produced were friable andwhitish and their growth patternfollowed a sigmoid shape. The deceleration phase started on the 23rdday of cultivation.Conclusion:Callus induction in leaf explants ofC. annuum... Mostrar Tudo |
Palavras-Chave: |
Callogenesis; Calogêneses; Growth curve; Metabolismo secundário. |
Thesagro: |
Curva de Crescimento; Pimenta. |
Thesaurus Nal: |
secondary metabolites. |
Categoria do assunto: |
-- |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/147697/1/Differentiation-SANTOS-2016.pdf
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Marc: |
LEADER 02419naa a2200217 a 4500 001 2053310 005 2016-10-06 008 2016 bl uuuu u00u1 u #d 100 1 $aSANTOS, M. R. A. dos 245 $aDedifferentiation of Leaf Cells and Growth Pattern of Calluses of Capsicum annuumcv. Etna.$h[electronic resource] 260 $c2016 520 $aBackground:In vitrocell suspension cultivation systems have been largely reported assafe and standardized methods for production of secondary metabolites with medicinaland agricultural interest.Capsicum annuumis one of the most widely grown vegetablein the world and its biological activities have been demonstrated against insects, fungi,bacteria and other groups of organisms. The determination of procedures for thededifferentiation of cells into callus cells and the subsequent study of the callus growthpattern are necessary for the establishment of cellsuspensions and also to subsidizestudies regarding the bioactivity of its secondarymetabolites. To date, no study hasdescribed the development of protocols for callus induction inC. annuumL. cv. Etna. Objective:The objective of this study was to establish a protocol for dedifferentiationof leaf cells of the cultivarC. annuumcv. Etna and to determine the growth pattern ofthe calluses with a focus on the deceleration phase, when the callus cells must besubcultured into a liquid medium in order to establish cell suspension cultivationsaiming at the production of secondary metabolites.Results:The treatment that resultedin the highest %CI, ACCC and callus weight was thecombination of 4.52 μ M 2,4-D +0.44 μ M BA. The calluses produced were friable andwhitish and their growth patternfollowed a sigmoid shape. The deceleration phase started on the 23rdday of cultivation.Conclusion:Callus induction in leaf explants ofC. annuumcv. Etnacan be achieved inMS medium supplemented with 4.52 μ M 2,4-D + 0.44 μ MBA, which results in highcellular proliferation; in order to start a cell suspension culture, callus cells on the 23rdday of culture should be used. 650 $asecondary metabolites 650 $aCurva de Crescimento 650 $aPimenta 653 $aCallogenesis 653 $aCalogêneses 653 $aGrowth curve 653 $aMetabolismo secundário 700 1 $aSOUZA, C. A. de 773 $tAustralian Journal of Basic and Applied Sciences$gv. 10, n. 12, p. 362-368, 2016.
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Embrapa Rondônia (CPAF-RO) |
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Registros recuperados : 187 | |
1. | | SANTOS, M. R. A. dos. Cultura de tecidos vegetais em Rondônia. Agrosoft, 2008. Meta 2008.
Artigo também publicado com o título: Micropropagação vegetal. Demais veículos em que o artigo foi publicado: Maxpress Net; Cultivar; Portal do Agronegócio; CREA-RO; O Guaporé; Boletim Pecuário; ClicNews; BragaRural;...Tipo: Artigo de Divulgação na Mídia |
Biblioteca(s): Embrapa Rondônia. |
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Registros recuperados : 187 | |
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